A DIRECT MICROBIOLOGICAL ASSAY FOR QUINOLINIC ACID
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چکیده
منابع مشابه
A MICROBIOLOGICAL ASSAY METHOD FOR p-AMINOBENZOIC ACID
Since the eshablishment of p-aminobenzoic acid as a member of the B vitamin group, a considerable interest has been shown in methods of determination in natural materials. Since known chemical methods are not sufficiently sensitive, it became evident that microbiological tests should be the most practicable. The organism Clostridium acetobutylicum has been used (I) but no general assay procedur...
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Brady, T. G. (1941). Biochem. J. 35, 855. Clark, V. M. & Kalckar, H. M. (1950). J. chem. Soc. p. 1029. Doudoroff, M., Barker, H. A. & Hassid, W. Z. (1947). J. biol. Chem. 168, 725. Friedkin, M. & Kalckar, H. M. (1950). J. biol. Chem. 184, 437. Hoff-J0rgensen, E. (1952). Biochem. J. 50, 400. Kalckar, H. M. (1947 a). J. biol. Chem. 167, 429. Kalckar, H. M. (1947b). J. biol. Chem. 167,445. Klenow,...
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Since the observation (1) that tryptophan and nicotinic acid are mutually interchangeable, within limits, in supporting the growth of rats, considerable evidence has accumulated which indicates that biosynthesis of nicotinic acid can proceed from tryptophan (2-5). Unequivocal evidence for this transformation was provided by the experiments of Heidelberger et al. (6) who fed labeled tryptophan @...
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Although vitamin B12 can be assayed biologically with mice, chicks or rats, the microbiological method of assay is preferred since it is more rapid and economical. One serious shortcoming of many microbiological B12 assay procedures is the lack of specificity when crude materials are assayed. Crude materials can contain inhibitory or stimulatory factors which cause serious errors in assay resul...
متن کاملPreservation of microbiological assay organisms by direct freezing.
'rhe effects of freezinig on bacterial cultures have been investigated by numerous workers. The most commonly used method of preserving microorganisms is lvophilization (drying from the frozen state), but this is a drastic procedure for in most instances comparatively few organisms (2 to 3 per cent) survive the freeze drying process. Cultures preserved in this way have remained viable for as lo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55959-7